Plasmid files mammalian expression vectors p3xflagcmv14. Highthroughput chemical screen identifies a novel potent. P3xflag cmv 10 expression vector, supplied by millipore, used in various techniques. Functional characterization of a short peptidoglycan recognition. Improved secretion of glycoproteins using an nglycan. Ectopic expression of necdin induces differentiation of mouse. Fkbp51 increases the tumourpromoter potential of tgfbeta. Vector for expression of nterminally 3xflagtagged proteins in mammalian cells. Either p3xflagcmv14, an expression vector purchased from. Vector with a neomycin resistance marker for expression of cterminally 3xflag tagged.
The pcr products were then excised, purified and subcloned into pjet1. Here, we investigated whether ndrg2 might be a 14q11 tumour suppressor. Functional characterization of il10 and its receptor. Huh7 cells were transfected with p3xflagcmv control and 1433. Loss of ndrg2 expression activates pi3kakt signalling via pten. Construction of bacterial and mammalian expression vectors for ydohh and hdohh. The vector encodes three adjacent flag epitopes asptyrlysxaaxaaasp downstream of the multiple cloning region. Louis, missouri, usa carrying the fkbp51 gene was transfected using metafectene biontex, munich, germany, according to manufacturers recommendations. The sequence alignment was performed using the clustal o software and edited with. Jie chens lab contains the insert lancl2 and is published in mol biol cell. The msms data were analyzed by the mascot server software matrix science, yokohama.
Band intensities on blots were quantified using nih image j software. In thermal shift assays, the first derivative of the fluorescence intensity. Cell surface expression of channel catfish leukocyte. For luciferase in p3xflagcmv14, 2 ng of expression vector with. The adpgrps1 fusion protein expressed in the hek293t cells was analyzed. The sequence alignment was performed using the clustal o software. For expression of the fviii and fv fulllength and deletion mutant proteins in mammalian cells, the codonoptimized fviii and fv genes were inserted. Construction, expression and kinase function analysis of. The pcr product was subcloned into p3xflagcmv14 vector sigmaaldrich. Home resources plasmid files mammalian expression vectors pflag cmv 2 pflag cmv 2 vector for expression of nterminally flagtagged proteins in mammalian cells. Moderate expression of adpgrp1 was also detected in muscle, heart, skin and lung figure 3.
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